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Crh-ires-cre

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WebFeb 2, 2024 · For manipulation of CRH neurons in the PVN, AAV10-hSyn-Flex-hM3Dq-mCherry was delivered bilaterally to the PVN of male CRH-ires-Cre mice (A/P, − 0.69 mm; M/L, ± 1.06 mm from the bregma; D/V, − 4.66 mm from the dura; with a 10° angle toward the midline in the coronal plane) through an internal cannula (Eicom Co., Kyoto, Japan) at a … Web012704 B6(Cg)- Crh tm1(cre)Zjh /J CRH-ires-CRE knock-in mice have Cre recombinase expression directed to CRH positive neurons by the endogenous promoter/enhancer … MGI Workshops. If you would like to host an MGI workshop at your institution, …

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WebOct 12, 2024 · CRH-ires-Cre B6(Cg)-Crhtm1(cre)Zjh/J mice (012704) were obtained from Jackson Lab (CA, USA), and were bred on a C57 BL/6 background for more than six … WebMay 6, 2024 · Crh-IRES-Cre; Ai14 tdTomato: Organism: Mus musculus: Characteristics: tissue: CRH-expressing neuron genotype: Crh-IRES-Cre; Ai14 tdTomato condition: ELA weight: 5 age at collection: P11 protocol: RNA library prep: Nextera XT DNA Library Prep Kit (FC-131-1096, Illiumina) Treatment protocol: jenis jenis obligasi

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WebMay 28, 2013 · Here we characterize CRH neurons in the PVN of mice that express tdTomato fluorophore, generated by crosses of recently developed Crh-IRES-Cre driver … WebConclusions: By using the CRH-IRES-Cre;Ai32 mouse line and fMOST imaging, we obtained region-specific morphological distributions of CRH neurons at the dendrite level in the whole mouse brain. Taken together, our findings provide comprehensive brain-wide morphological information of stress-related CRH neurons and may WebCRH-IRES-Cre, Crh:FLOX, and VIP-IRES-Flp mice were purchased from Jackson Laboratory (012704, 030110, 016962, 028578). Oxtr-Cre mice were generated by The Rockefeller University GENSAT Project. CRH … jenis jenis office

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WebIn the medulla, the inferior olivary nucleus specifically expresses Cre in Crh-IRES-Cre (ZJH) mice (Taniguchi et al., 2011) and the dorsal cochlear nucleus has anatomically restricted expression of Cre in the Pnmt-Cre line (Ebert et al., 2004). Finally, several Cre lines show expression in the cerebellum, including Purkinje cells (e.g., Pcp2 ... WebJun 4, 2014 · In the CRH-ires-Cre driver line, 92% of the Cre-positive cells were located in the GCL, and 8% were in the INL (Fig. 1C, n = 572 cells from 8 retinas). Most of the labeled cells in the GCL had small, brightly … jenis jenis objek wisataWebJan 21, 2024 · The used Crh-IRES-Cre;Ai9 animals −in which Cre mediated recombination [10.] resulted in tdTomato fluorophore expression in Crh neurons −were derived from second crosses of homozygous Crh-IRES-Cre/Ai9 genotypes. Mice were bred at the specific pathogen free (SPF) level and were maintained at the minimal disease (MD) … jenis jenis online travel agent

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Crh-ires-cre

Transgenic Characterization :: Allen Brain Atlas: Mouse …

WebTo address the role of CRH in cortical microcircuits and identify which cell types express CRH in the mPFC, we employed the knockin CRH-IRES-Cre mouse line (B6(Cg)-Crh tm1(cre) Zjh /J, JAX ... WebOct 1, 2024 · We used in vivo fiber photometry to record cell-type-specific Venus fluorescence as a readout of Per2 transcriptional activity in the PVN of heterozygous Crh …

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WebMar 15, 2024 · Comparison of the morphological features of CRH neurons in three fluorescent-reporter mouse lines. Comparisons of the distributions and morphologies of … WebMar 15, 2024 · By using the CRH-IRES-Cre;Ai32 mouse line and fMOST imaging, we obtained region-specific morphological distributions of CRH neurons at the dendrite level …

WebApr 1, 2024 · First, on day 1, we injected CRH-ires-Cre mice with two Cre-inducible helper viruses, which consisted of an adeno-associated virus (AAV) encoding RV glycoprotein (RG) and a separate virus expressing avian-specific retroviral receptor (TVA-GFP). TVA-GFP and RG were required for rabies transduction. Two weeks later, on day 14, the mice were ... WebSep 16, 2024 · TRAP translational profiling is a method that allows investigators to genetically characterize specific cell types in complex tissues such as mouse brain. Using this technique we obtained RNA-Seq data from actively translating transcripts present in neurons in the prefrontal cortex of adult Crh-IRES-Cre mice (Jackson 012704).

WebOct 12, 2024 · CRH-ires-Cre B6(Cg)-Crhtm1(cre)Zjh/J mice (012704) were obtained from Jackson Lab (CA, USA), and were bred on a C57 BL/6 background for more than six generations. Male offspring at 6–12 weeks of age were used in the experiments, and randomly assigned to groups. Genotypes were determined by polymerase chain reaction … Webwe characterize CRH neurons in the PVN of mice that express tdTomato fluorophore, generated by crosses of recently developed Crh-IRES-Cre driver and Ai14 Cre-reporter mouse strains. tdTomato containing PVN neurons in Crh-IRES-Cre;Ai14 mice are readily visualized without secondary-detection methods. These neurons are predominantly …

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WebSep 18, 2024 · Crh-IRES-Cre;Ai14 mice were derived from crosses of the Crh-IRES-Cre and Ai14 genotypes. Male mice at 7–12 weeks of age were used for the experiment. Mice were randomly selected. Mice from one litter were spread across experimental groups. Age and body weight were matched for each experiment. Mice were group housed (maximum … jenis jenis on the job trainingWebJul 25, 2024 · Here, we performed a comprehensive study of CRH neurons in CRH-IRES-Cre;Ai3 mice via automatic imaging and stereoscopic cell counting in a whole mouse brain. We acquired four datasets of the CRH distributions with co-localized cytoarchitecture at a voxel resolution of 0.32 μm × 0.32 μm × 2 μm using brain-wide positioning system (BPS). lakesia para frieiraWebFeb 27, 2024 · A projection of CRH/GABA neurons in the medial BLA to the medial NAc shell. a–c Retrograde tracing of CRH + neuronal inputs to medial NAc shell of CRH-ires-CRE mice. a Schematic of construct and ... jenis jenis operator aritmatikaWebPrepared by: Child Trends 4301 Connecticut Avenue, N.W., Suite 350 Washington, DC 20008 Subcontractor to: Mathematica Policy Research 600 Maryland Ave., S.W., Suite 550 jenis jenis operasi bisnisWebMay 28, 2013 · Pairs of either homozygous Crh-IRES-Cre or Ai14 genotypes were mated, and the resulting heterozygous Crh-IRES-Cre;Ai14 male offspring used in subsequent experiments. For stress experiments, … jenis jenis obat bebasWebFeb 2, 2024 · Chronic activation of CRH PVN neurons promotes tumor progression through the excess secretion of glucocorticoids. A Schematic images of the specific activation of CRH PVN neurons by the injection of AAV-derived transgenes into the PVN of CRH-ires-Cre mice. Schematic illustrations of CRH-ires-Cre mice (A-i), AAV injection site (A-ii) … lakesia para unhasWebCrh-IRES-Cre_ZJH. Z. Josh Huang. Scattered expression in cortex. Enriched expression in piriform cortex, amygdala, hypothalamus, and thalamus. Ctgf-T2A-dgCre. Allen Institute … lakesia para unhas preço