WebHoxb8 is a homeobox gene that inhibits myeloid differentiation. Integration of a murine stem cell virus encoding HoxB8 under the control of mutant estrogen receptor promotes … Web29 nov. 2024 · Serial monitoring of peripheral blood demonstrated that Hoxb8-FL derived cells had disappeared by 4 weeks, and did not reappear. However, at 6 months CEBPA …

HOXB8 – Joint Biology Consortium

BM– and Hoxb8-FL–derived DCs and macrophages were generated by cultivating unfractionated BM cells or Hoxb8-FL cells for 6 d in standard growth medium (RPMI 1640 (Invitrogen) for DCs or DMEM (Invitrogen) for macrophages, supplemented with 10% (v/v) FCS (Hyclone), 50 mM 2-mercaptoethanol and … Meer weergeven Antibodies used were against B220 (RA3-6B2, dilution 1:200), CD4 (RM4-5, dilution 1:200), CD45.1 (A20, dilution 1:200), MHC class II … Meer weergeven BM cells were collected by flushing femurs of 4–8-week-old female C57BL/6J or B6.SJL mice with 10 ml RP-10 (RPMI 1640 … Meer weergeven The plasmids MSCV-ERHBD-Hoxb8 and the ecotropic packaging vector pCL-Eco (Imgenex) were both transfected into HEK293T … Meer weergeven Hoxb8-FL cells and BM cells were differentiated in the presence of M-CSF contained in L-cell conditioned medium for 7 d, followed by incubation with FITC-labeled IgG-coated beads (1:100, Cayman Chemical, … Meer weergeven fs2020 gatwick airport

Hematopoietic progenitor cell lines with myeloid and …

WebHoxb8 cells are murine hematopoietic progenitor cells that can be efficiently differentiated into neutrophils and macrophages resembling their primary counterparts. Web1 jan. 2024 · Flow cytometry and single-cell transcriptomic characterisation of cells at different time points revealed that the mutation stalls the multipotent HOXB8-FL cell line along the differentiation trajectory resulting in a self-renewing progenitor-like state exhibiting haematopoietic markers indicative of a plasmacytoid dendritic cell-like phenotype. WebTo test if Cas9-Hoxb8 cell-derived DCs rely on the same mechanisms and thus can be exploited as a tool to study DC migration, we used lentiviral-based CRISPR/Cas9 … fs2020 fbw a320 flight plan