Reading rfu
Webfor 2 hours prior to reading the fluorescence on Varioskan LUX. A. Data was plotted as Relative Fluorescence Values (RFU) versus the log. concentration of blasticidin. B. Data from plot A was normalized for baseline correction and plotted as percent survival versus the log. concentration of blasticidin.
Reading rfu
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WebAug 23, 2016 · An approach to gain setting that I like to use is to set the gain based on the sample with the most fluorescence in order to get about 30-50% of the maximal signal before the detector saturates ...
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