Splet05. okt. 2024 · The primary antibodies used for the Western blot analyses were as follows: 14-3-3ζ (Santa Cruz Biotechnology, SC-293415, diluted at 1:500), E-cadherin (Abcam, ab133597, diluted at 1:5,000), N-cadherin (Abcam, ab76057, diluted at 1:1,000), vimentin (Abcam, ab137321, diluted at 1:1,500), BMP2 (Abcam, ab214821, diluted at 1:1,000), … SpletWestern blot analysis indicated that the protein levels of PSMD10 in the PSMD10-shRNA MM cells were significantly lower than those in the cells transduced with the negative …
MISSION® Lentiviral Controls - Sigma-Aldrich
Splet05. feb. 2024 · A, B Quantitative RT-PCR analysis of the WT1 expression level in the IHH4 cells and BCPAP cells treated with WT1 shRNA. C, D Western blot assay showing the knockdown efficiency of WT1 in IHH4 cells and BCPAC cells treated with lentiviral WT1 shRNA. E, F CCK-8 assay indicating the proliferation of IHH4 and BCPAC cells upon WT1 … SpletAbstract. Small hairpin RNA (shRNA) is a powerful tool for inhibiting gene expression. One limitation has been that this technique has been used primarily to target a single gene. … iowa state social studies standards
Lentiviral Transduction Protocol - Sigma-Aldrich
Splet19. okt. 2024 · Western blot testing the mitochondrial fraction lysates confirmed specific depletion of YME1L protein in the mitochondria of P1 human glioma cells by the applied shRNA and KO strategies . While its expression was not detected in mitochondria-null lysates of P1 glioma cells with or without the applied genetic treatments . SpletAvailable TRC shRNA controls include the pLKO.1 empty vector as a negative control and an eGFP shRNA as a positive control. Utilizamos cookies propias y de terceros para mejorar nuestros servicios y mostrarle publicidad relacionada con sus preferencias mediante el análisis de sus hábitos de navegación. SpletFigure Legend Snippet: A, Western blot analysis of TMEM16A protein expression in SW620 cells that were transfected with shRNA #1 and #2. B, The bar graph summarizes the relative expression level of TMEM16A protein from panel A. Expression of TEMEM16A protein was normalized with the expression level of β-actin (n = 3). iowa state social science courses