T4 ligase m0202m
WebSource of Protein: A recombinant E. coli strain carrying the cloned T4 DNA Ligase gene. Unit Definition: 1 unit is defined as the amount of DNA Ligase required to join 50% of 100 ng of DNA fragments with cohesive termini in 50 µl 1X DNA Ligase Buffer following a 30 minute incubation at 23°C. Molecular weight: 55,292 Daltons. Quality Control ... WebT4 DNA Ligase Packaging Unit: 100.000 units € 288,00 -+ Add to Cart M0202M T4 DNA Ligase, conc. Packaging Unit: 100.000 units € 288,00 -+ Add to Cart Login Register …
T4 ligase m0202m
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WebE. coli DNA Ligase. OEM by QIAGEN offers bulk manufacturing of E. coli DNA Ligase in custom formulations. T4 DNA Ligase MBG. For joining both blunt-ended and cohesive … WebThis product can be used for the following applications: Cloning of restriction fragments Joining linkers and adapters to blunt-ended DNA
WebT4 ligase, including 10× ligase buffer (New England Biolabs, catalog number: M0202M) 23. 100 mM ATP (GE Healthcare, catalog number: 27-2056-01) 24. 0.5 M EDTA, disodium salt (Sigma-Aldrich, catalog number: E5134) 25. 5 M NaCl (Sigma-Aldrich, catalog number: S9888) 26. Sepharose 4B size exclusion chromatography resin (GE Healthcare, catalog WebNCBI's Gene Expression Omnibus (GEO) is a public archive and resource for gene expression data.
WebManufacturer: New England Biolabs, Inc. M0202M The industry standard for performance and quality. T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5' phosphate and 3' hydroxyl termini in duplex DNA or RNA. Catalog No. 50-811-604 $260.00 / Each of 1 Qty Check Availability Add to cart WebTraditionally, a ligation reaction (blunt or cohesive ends) using traditional T4 DNA Ligase involves incubation at 16°C using 0.1-1 µM DNA (5´ termini) in 1X T4 DNA Ligase Buffer. For your convenience, T4 DNA Ligase can also be used at room temperature, and is available in concentrated form .
WebNEB M0202M, 2,000,000Units/mL, 100,000 units. (50 µL per tube, calculate the number of tubes you need, 2 plates = 40 µL T4 ligase vol.) ... 5.0 µL T4 DNA Ligase Buffer (10X) 1000 µL T4 DNA ...
Webcontrol performed without T4 DNA ligase in the first ligation reaction. 2. Wash cells with PBS (-/-) 3. Detach cells using trypsin (e.g. HeLa/U2OS) or accutase (e.g. NHE Ks) 4. Add an equal amount of complete growth medium to the detached cells 5. Transfer cells to a Falcon tube and centrifuge at 1300 rpm for 5 min at room temperature to pellet ... qcustomplot drawtexthttp://www.enzymatics.com/wp-content/uploads/2014/12/L6030-LC-L-REV-F-T4-DNA-Ligase-PSF-EFF04SEP2014.pdf qcut binning error not enough valuesWebT4 DNA Ligase can be used to efficiently join blunt and cohesive ends and for the repair of single stranded nicks in duplex DNA, RNA or DNA/RNA hybrids. The enzyme catalyzes the formation of a phosphodiester bond between the terminal 5′ phosphate and a 3′ hydroxyl groups of duplex DNA or RNA. T4 DNA Ligase by Enzymatics is supplied in a ... qcustomcalendarwidgetWebUsed in Golden Gate Assembly; maintains high activity levels in T4 DNA Ligase Buffer often used in this application Type IIS restriction enzymes recognize asymmetric DNA sequences and cleave outside of their recognition sequence 100% activity in rCutSmart Buffer Time-Saver™ qualified for digestion in 5-15 minutes Reduced star activity qcustomchartWebThe mixture of 10uM Forward primer with 5’ end, 10X T4 DNA ligase buffer, T4 PNK (10 units) and Nuclease-free water 1) incubated at 37°C for 1 hour, and 2) incubated at 65°C for 20 minutes to ... qcut in pandasWebBlunt/TA Ligase Master Mix ends (about 1 μg of a 3 kb plasmid) and incu- #M0367S 50 rxns bate at 37°C for 30–60 minutes. Endonuclease Activity: Incubation of a 50 µl #M0367L 250 rxns 4. Purify DNA by gel purification, spin-column or reaction containing 50 units of CIP with 1 μg of phenol extraction. φX174 RF I DNA for 4 hours at 37°C ... qcustomplot opengl加速WebT4 DNA Ligase requires ATP as a cofactor. Highlights • Active in Themo Scientific restriction enzyme, PCR, and RT buffers (when supplemented with ATP) • Fast—sticky-end ligation is completed in 10 minutes at room temperature • Supplied with PEG solution for efficient blunt-end ligation Applications qcut buffer