Topflash or fopflash
Web26. dec 2024 · topflash is a tcf reporter plasmid containing two sets of three copies of wild-type tcf binding sites driven by the thymidine kinase minimal prompter and upstream of … WebFOPflash (mutant TCF binding sites) 21-169 Sigma-Aldrich FOPflash (mutant TCF binding sites) Transfection grade T cell factor (TCF) reporter plasmid containing 2 full & one incomplete copy of the Tcf binding site (mutated) followed by 3 copies in the reverse orientation. Serves as a negat More>>
Topflash or fopflash
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Web17. okt 2024 · Using TOPFlash/FOPFlash ratio to examine the effect of MYC on the activity of the WNT pathway, we found that DLD1 colon cancer cells transfected with TOPFlash … WebThis 134bp fragment was gene synthesized and cloned into the ASP718 and XMA-1 sites of the vector. Note: This plasmid was published as M51 Super 8x FOPFlash, but the plasmid …
Web我因为刚刚接触，用的是分别转染TOPflash和FOPflash，但遇到了问题。 2、FOPflash值不稳定，并不像有些文献里的那样，各组之间FOP值都不变，我的有时变化2-3倍。 而且FOP值很高，同样量的TOP和FOP，FOP值远高于TOP，除非激活剂强刺激才使TOP/FOP值达到个位数。 3、有个 基因 ，做了几次，发现TOP值没怎么升高，而FOP值降的挺厉害，导 … Web19. jan 2024 · A, B) Cells were transfected with TK-renilla and TOPflash or FOPflash in the presence of A) FLAG-DVl1 or C) GFP-FZD5 and HA-LRP6, or appropriate vector controls. A) 1-way ANOVA ( n = 9; F = 146.199, p < 0.001) followed by Bonferroni post-hoc analysis revealed increased DVL1-driven TOPflash activity in Lrrk2 knockout cells ( p < 0.001 …
WebTOPFlash/FOPFlash assays. TOPFlash is a luciferase reporter that contains a minimal fos promoter coupled to Tcf-binding sites upstream of a modified firefly luciferase gene. … Web5. jún 2024 · a HEK293T cells were transfected with plasmids encoding Topflash (β-catenin-responsive firefly luciferase reporter) or Fopflash (mutant form of Topflash that cannot be activated by β-catenin ...
WebA) DLD-1 cells were transfected with TOPflash or FOPflash reporter vectors and pRL-CMV together with empty vector (CT) or pCI-βcatS37A, the mutated β-catenin expression vector …
Web16. mar 2024 · TOPflash/FOPflash luciferase assay Cells (5 × 10 3 ) were seeded into a 96‐well plate, and the next day, each well was transfected with a total of 50 ng DNA: 35 ng of KAR32 MSCV_C_HA_FLAG UBE2T expression plasmid or empty vector control, 10 ng of TOPflash or FOPflash, and 5 ng of β‐galactosidase plasmid (control for transfection ... horas de juego zelda breath of the wildWebAs shown in figure 2, ENU-T1 cells showed lower activation of wnt signalling (TOPflash/FOPflash ratio 1,38 (σ: 0,47)) than 293T cells [46] which are known to have a low intrinsic wnt-activity... loons on lake soundsWeb1 From what I can understand, TOPflash is a luciferase reporter plasmid that contains two sets of 3 copies of the wild-type TCF binding regions. If the canonical Wnt signaling is activated, beta-catenin will translocate to the nucleus to associate with TCF/LEF … horas en chinaWebTopflash or Fopflash control was transfected into A7r5 cells along with expression constructs for β-catenin, IL2R-Fat1 IC , N-cadherin, and/or IL2R-E-cadherin IC . The … horas em orlyWeb10 votes, 19 comments. I want to play a 5v5 with my friends and we can't anymore because popflash doesnt work anymore for some reason. Do you guys… loons of north americahttp://www.damor.cn/question/2173 loonsom invoicesWebAfter 48 hours, the TopFlash/FopFlash luciferase activity of CD133 + and CD133 - Huh7 cells was measured. Values shown are relative luminescence. ( H ) Huh7 cells were treated … loons ontario